The RAPD technique has been successfully used in a variety of taxonomic and genetic diversity studies. The genetic diversity of 19 accessions of Salvadora oleoides collected from different regions of North-West India has been reported in this paper using 16 RAPD primers. 164 bands were scored corresponding to an average of 10.25 bands per primer with 146 bands showing polymorphism (90.09%). Jaccard similarity coefficient ranged from 0.21 to 0.94. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters CL1 and CL2. The dendrogram has put all the genotypes in two clusters CL2 comprises of two accessions (Jalore & Jhunjhunu) while CL1 is further divided into two groups (1 & 2). The group 1 comprises 4 accessions (Rohtak, Hisar, Rewari, M. Garh) while group 2 is further divided in two sub groups (SG1 & SG2) out of which SG1 comprises 6 accessions while SG2 comprises 7 accessions. The study revealed rich genetic diversity among S. oleoides of North-West India.