Comparative modeling remains the most dependable and routinely used method for protein structure predictions. The pmpM gene belonging to MATE family of transporters of Pseudomonas aeruginosa, an opportunistic nosocomial pathogen in humans, is a prime target sequence responsible for conferring multidrug resistance through H(+)/drug antiporter efflux pumps. Its structure elucidation is necessary to analyze its functional characteristic which makes it resistant to many known antibiotics. In this study we have reported a 3D-structure predicted using homology modelling software Modeller 9v8. The structural validation was done in RAMPAGE, energy minimization was performed in ANOLEA and the model was finally verified in PROCHECK. The secondary structure, clefts and domain analysis was done using different bioinformatics tools. Thus various computational analyses will help in uncovering its possible activity within the target sites in human and design novel drugs based on their active site analysis.