Enzyme activity and biochemical constituents of healthy and stem rot infected tissues of susceptible variety of groundnut TMV2 were evaluated. Results revealed that higher activity of Oxidative enzymes (catalase, polyphenol oxidase and peroxidase) were present in infected leaves than healthy ones. In case of Biochemical constituents, higher amount of total and Ortho-di hydric phenols and protein content was observed in infected plants as compared to healthy plants. Higher content of sugars and chlorophyll was observed in healthy plants as compared to infected plants.
Comparative modeling remains the most dependable and routinely used method for protein structure predictions. The pmpM gene belonging to MATE family of transporters of Pseudomonas aeruginosa, an opportunistic nosocomial pathogen in humans, is a prime target sequence responsible for conferring multidrug resistance through H(+)/drug antiporter efflux pumps. Its structure elucidation is necessary to analyze its functional characteristic which makes it resistant to many known antibiotics. In this study we have reported a 3D-structure predicted using homology modelling software Modeller 9v8. The structural validation was done in RAMPAGE, energy minimization was performed in ANOLEA and the model was finally verified in PROCHECK. The secondary structure, clefts and domain analysis was done using different bioinformatics tools. Thus various computational analyses will help in uncovering its possible activity within the target sites in human and design novel drugs based on their active site analysis.
Thirty isolates of Sclerotium rolfsii, the causal agent of stem rot of groundnut obtained from different Rayalaseema areas of Andhra Pradesh, India were categorized into four groups, on the basis of oxalic acid production in the culture filtrate and severity of patrhogenicity on groundnut seedlings. The group four was more dominant than other groups and highly virulent to groundnut seedlings. There was a positive correlation between oxalic acid production and the virulence of the isolate.