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Toxicity of crude extracts of Ocimum suave leaf oil on cowpea weevil (Callosobrochus maculatus) (f) (coleoptera; bruchidae) and test of cowpea seeds’ viability.

April 7, 2014 · Volume 3; Issue 1

Toxicity of Ocimum suave (Wild basil) crude oil extract on C. maculatus and test on seeds’ viability, was determined in the laboratory using contact toxicity assay method of different concentration 0.02, 0.04, 0.06 and 0.08mg to 20ml of water and 0.00g/20ml of water (control). 20g of cowpea seeds were weighed into separate Petri dishes and 10 adult weevils were inoculated and replicated four times. Each treatment was observed for 96h and recorded after every 12h. The result indicated that the crude oil extract had significant difference (p0.05) in the germination of treated cowpea seeds (Vigna unguiculata L) compared to the untreated control, which shows that the seeds still remained viable. This result showed that O. suave crude oil is effective in the protection of cowpea against cowpea bruchid Callosobruchus maculatus.

Tree species with potential of nitrogen fixation in agroforestry system adopted by farmers in semi-arid region of Southern India

April 7, 2014 · Volume 3; Issue 1

In semi arid zone of southern Tamilnadu, India farmers are recognized that planting with multipurpose nitrogen fixing trees including medicinal plants in an agroforestry system for sustainable income generation.To identify the existing agroforestry system. A systematic survey was carried out to find out the nitrogen fixing trees planted in the agroforestry system revealed that a total of economically important 9 tree species belonging to 8 genus of 3 families were commonly cultivated without specific model. These multipurpose usable trees improve soil physical properties, maintain soil organic matter, fix the atmospheric nitrogen and promote nutrient cycling through high amount of litter production, which increase the main crop yield.

Physicochemical parameters and heavy metal content of water, fish and sediments from cross river at afikpo north local Government area of Ebonyi state, Nigeria

April 7, 2014 · Volume 3; Issue 1

Physicochemical parameters and heavy metal content of the Cross River at Afikpo North Local Government Area were determined using standard analytical methods. Four sampling stations were selected along the river and sampled during March to October, 2004. Parameters determined include chloride, conductivity, salinity, ammonium ion, turbidity, pH, dissolved oxygen (DO), biological oxygen demand (BOD5) and total hardness as well as concentration of some heavy metals (Fe, Pb, Cd, Cu, Ni and Zn) in both water, fish and sediment samples. Concentration of heavy metals was determined by atomic absorption spectrophotometry while Na and K were estimated by flame photometry. The river was slightly acidic in Station 1 (pH 6.80) and slightly alkaline in Station 4 (pH 7.47). Turbidity ranged from 16 FTU (in Station 4) to 22.0 FTU (in Station1) with a mean value of 19.250 FTU. The dissolved oxygen range of 5.16 (Station 2) to 8.0 (Station 4) ensures the survival of aquatic organisms. Iron was the most abundant metal both in sediment (99.78 mg/kg), fish (11.45 mg/kg) and water (4.85 mg/L). Cadmium had the lowest concentration in water (0.19 mg/kg) while Zn and Ni had the lowest concentrations in fish (0.18 mg/kg) and sediment (1.55 mg/kg), respectively. Generally the heavy metals had significantly higher (p

Larvicidal efficacy of Citrullus colocynthis, Leucas aspera, Strychnos nux-vomica against Culex quinquefasciatus

April 3, 2014 · Volume 3; Issue 1

Crude extracts of three plants Citrullus colocynthis, Leucas aspera, and Strychnos nux-vomica were screened for their larvicidal activities against the third instar of Culex quinquefasciatus. The mortality rate of the mosquito larvae was evaluated against the three botanicals at different periods of exposure (24h, 48h, and 72h). Hexane, Chloroform and Ethyl acetate were used as solvents for the crude extracts. C. colocynthis showed the maximum larvicidal effect (100% mortality) in 750 ppm and 1000 ppm in hexane extract at 24h period of exposure. L. aspera also showed the same activity in hexane extract at 24 h, while S. nux-vomica showed slightly lower effect.

Effect of monocrotophos on the enzyme activity of fresh water fish Ctenopharyngodon idella (grass carp)

April 3, 2014 · Volume 3; Issue 1

Pesticide pollution in aquatic environment brings about sudden and drastic changes in aquatic organisms. The indiscriminate use of pesticides in agricultural operations affects the aquatic environment to a greater extent. Pesticides like endosulphan and monocrotophos which are widely used in agriculture affect fish population drastically. In the present study the effect of monocrotophos on the enzymes Acid Phosphatase, alkaline phosphatase and lactate dehydrogenase of Ctenopharygodon idella has been estimated. The activity was found to be decreased significantly due to Pesticide intoxification.

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  • A study on acute toxicity, behaviour, growth, somatic indices and oxygen consumption in Indian flying barb, Esomus danricus (Hamilton-Buchanan) on exposure to organophosphate pesticide, malathion (EC50) May 1, 2014
    Acute toxicities of commercial grade malathion (EC50) on Esomus danricus were determined by static-with-renewal tests and found to be 0.026, 0.022, 0.02 and 0.017 mgl-1 respectively for 24, 48, 72 and 96 hours. During acute toxicity, the normal colour was found to fade, along with copious mucous secretions, irregular, erratic and jerky movements. Three sub […]
    Suchismita Das
  • Assessment of genetic diversity using RAPD marker among different accessions of Salvadora oleoides of North-West India May 1, 2014
    The RAPD technique has been successfully used in a variety of taxonomic and genetic diversity studies. The genetic diversity of 19 accessions of Salvadora oleoides collected from different regions of North-West India has been reported in this paper using 16 RAPD primers. 164 bands were scored corresponding to an average of 10.25 bands per primer […]
    Yadav J P
  • Cardioprotective effect of kolaviron (a Garcinia kola seed extract) in cholesterol-fed rats. May 1, 2014
    Flavonoids – a group of polyphenolic substances are naturally present in vegetables, fruits, seeds and beverages such as tea and wine. Studies have shown that flavonoid intake is inversely correlated with mortality from coronary heart diseases and myocardial infaction. The effect of kolaviron (a flavonoid complex) extracted from garcinia kola seeds on the plasma and […]
    Nwaneri Chidozie V O
  • Karyomorphology of two major carps, Catla catla and Labeo rohita May 1, 2014
    A detailed study of chromosomes of two species of Indian inland fish revealed the chromosomal number and type. It describes the relation between the two species. Their peculiar morphological characters have been recorded. These karyological observations provide strong evidence to conclude that the two species are closely related and have phylogenetic links.
    Nandini S
  • Free radical scavenging activity of Salacia chinensis root extract in streptozotocin-induced diabetic rats May 1, 2014
    This study was undertaken to evaluate the antioxidants property in the methanolic root extract of Salacia chinensis in streptozotocin (STZ) induced diabetic rats. The methanolic root extract of S. chinensis was administered orally at a dose of 250-mg/kg weight/day (28 days) to STZ-induced diabetic rats. The extract treated diabetic rats were significantly decreased the levels […]
    Periyar Selvam S
  • Role of oxidative enzymes and biochemical constituents in imparting resistance to groundnut (Arachis hypogaea L.) against stem rot disease caused by Sclerotium rolfsii April 7, 2014
    Enzyme activity and biochemical constituents of healthy and stem rot infected tissues of susceptible variety of groundnut TMV2 were evaluated. Results revealed that higher activity of Oxidative enzymes (catalase, polyphenol oxidase and peroxidase) were present in infected leaves than healthy ones. In case of Biochemical constituents, higher amount of total and Ortho-di hydric phenols and […]
    Reddy M N
  • Homology modelling and in-silico analysis of multidrug resistant protein, pmpm of Pseudomonas aeruginosa April 7, 2014
    Comparative modeling remains the most dependable and routinely used method for protein structure predictions. The pmpM gene belonging to MATE family of transporters of Pseudomonas aeruginosa, an opportunistic nosocomial pathogen in humans, is a prime target sequence responsible for conferring multidrug resistance through H(+)/drug antiporter efflux pumps. Its structure elucidation is necessary to analyze its […]
    Tarali Chowdhury
  • Variation in oxalic acid production by groundnut isolates of Sclerotium rolfsii April 7, 2014
    Thirty isolates of Sclerotium rolfsii, the causal agent of stem rot of groundnut obtained from different Rayalaseema areas of Andhra Pradesh, India were categorized into four groups, on the basis of oxalic acid production in the culture filtrate and severity of patrhogenicity on groundnut seedlings. The group four was more dominant than other groups and […]
    Saraswathi M
  • Toxicity of crude extracts of Ocimum suave leaf oil on cowpea weevil (Callosobrochus maculatus) (f) (coleoptera; bruchidae) and test of cowpea seeds’ viability. April 7, 2014
    Toxicity of Ocimum suave (Wild basil) crude oil extract on C. maculatus and test on seeds’ viability, was determined in the laboratory using contact toxicity assay method of different concentration 0.02, 0.04, 0.06 and 0.08mg to 20ml of water and 0.00g/20ml of water (control). 20g of cowpea seeds were weighed into separate Petri dishes and […]
    Ojianwuna C C
  • Tree species with potential of nitrogen fixation in agroforestry system adopted by farmers in semi-arid region of Southern India April 7, 2014
    In semi arid zone of southern Tamilnadu, India farmers are recognized that planting with multipurpose nitrogen fixing trees including medicinal plants in an agroforestry system for sustainable income generation.To identify the existing agroforestry system. A systematic survey was carried out to find out the nitrogen fixing trees planted in the agroforestry system revealed that a […]
    Rajendren K

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