The present study was carried out to assess the antibacterial activity of bacteria associated with Polychaetes. Polychaetes were collected from the coastal environment and the bacterial communities associated with the surfaces were isolated using traditional culture method. Four biofilm bacteria (Galionella sp., Alteromonas sp., S.aureus, Klebsiella sp.) isolated from the marine water was used as target organism for screening. Three surface associated bacteria were isolated from the surface. The EPS of the three surface associated bacteria were isolated and tested for their antimicrobial activity. The results showed that higher activity against Alteromonas sp. The bioactive compounds were separated by thin layer chromatography. The results showed higher activity against Alteromonas sp. Based on the study, it can be concluded that bacteria associated with Polychaetes would serve as a potential source for the isolation of bioactive compounds.