Morus alba L. is an economically important plant used extensively in sericulture, also possessesing medicinal properties. In vitro culture provides an effective means to overcome the common problems encountered in conventional propagation by stem cuttings and seeds. Effect of phytohormones, growth adjuvants, sugars and essential minerals on the in vitro response of M. alba explants has been described in the study. Phytohormones and growth adjuvants resulted in explicit response in terms of organogenesis. Sucrose and glucose containing culture media elicited best response among sugars in nodal explants. Typical symptoms were exhibited by the growing shootlets when the medium was deprived of essential minerals such as nitrogen and sulphur.