In recent years, medical science has turned to a variety of new products and technologies to halt the spread of infections. The most effective and promising antimicrobial agents being embraced by modern medical science today is silver, which is also one of the oldest. In 1940s breakthroughs in antibiotics such as penicillin, actinomycin and streptomycin, medical science began to shift its attention away from preventative compounds such as antimicrobial silver and focused instead on antibiotic treatments. With increasing drug-resistance and growing concern regarding the over-prescribing of antibiotics, there has been a resurgent interest in the use of antimicrobial silver. Unlike antibiotics, silver appears to be immune to resistance. Thus, the conjugation of antibiotic with AgNP (Silver nanoparticles) would prevent development of resistance by microbes and enhance the antimicrobial property of the antibiotic. This study is based on the synergistic effect of Cephalexin antibiotic with AgNP. The nanoparticles were evaluated for their increased antimicrobial activities with Cephalexin antibiotic against E.coli and S. aureus. The antibacterial activity of Cephalexin was increased in the presence of AgNPs against test strains. The results showed that the combination of antibiotics with AgNPs have better antimicrobial effects.
The impact of various clinical pathogens on the gut toxicity has remained an elusive question as the results have been highly inconclusive till date. The mice were classified as normal and test with difference in their feeding cycle. Various clinical pathogens used were Salmonella typhi, Shigella and Escherichia coli. The introduction of the clinical pathogens was followed by the removal of various visceral organs including small intestine, liver, spleen and kidney. Microbiological analysis of these organs using agar media, employing standard streak method yielded both qualitative and quantitative details about the toxicity caused by clinical pathogens used in these albino mice. Small intestine was found to having the highest accumulation of the pathogens in comparison with other visceral organs with 89 x 10-3 CFU. A clear correlation was found between the gut toxicity and the nature of the clinical pathogen employed. The study can be extended to the mechanism beneath the differences arising in the various visceral organs when encountered with clinical pathogens.
Radiation is used extensively in the fields of oncology, tissue engineering and therapeutics. Although radiation therapy is highly successful in the latter stages of benign and malignant tumour for its treatment, the side effects associated with the radiation are profound. The study was aimed at studying the impact of cobalt-60 irradiation on the tissue damage and cell deformation using albino mice model. Albino mice were grouped and irradiated with a cobalt-60 source. The grouping done was on the basis of radiation being passed. Test group of mice was irradiated with cobalt-60 source originating radiation. Control group of mice was not irradiated at all. The relationship between the tissue damage and amount of radiation passed was found to be directly proportional and in the case of cell deformation, it became constant after a point of time. Further studies can explain the differences arising between the tissue damage and cell deformation when radiation is passed through a cobalt-60 source.